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New Single-Cell Whole Genome Sequencing Workflow Allows Discovery of Novel Cellular Heterogeneity

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Fluidigm Corporation (NASDAQ:FLDM) recently announced a new single-cell application for its C1 system that improves whole genome sequencing of individual cells. Single-cell whole genome sequencing allows researchers to explore mutations in regulatory and protein coding regions of the genome in order to identify novel signatures within heterogeneous cell populations. This application will empower cancer researchers to discover and define specific mutation profiles to predict cancer susceptibility, metastasis, and therapeutic efficacy.

“It is essentially impossible to understand the distribution of mutations, rearrangements and copy number changes among cells through bulk sequencing,” said Gajus Worthington, Fluidigm President and Chief Executive Officer. “We have made single-cell DNA sequencing accessible, and believe that this can usher in a whole new phase of discovery in single-cell biology.”

Researchers are profiling genetic signatures of tumors and other complex diseases to identify the unique cell lineages driving disease onset. These diseases are often driven by somatic mutations that originate in individual cells and create genetically diverse clonal populations. As mutated cells proliferate, they generate complex polyclonal networks that may lead to the onset and recurrence of disease and the failure of therapies.

Traditionally, sequencing of bulk samples has been used to identify mutations across whole populations of cells within diseased tissues. However, it is impossible to associate mutation profiles to specific cells and identify genetic traits responsible for aberrant cell behavior.

With single-cell whole genome sequencing, researchers can get the whole picture. The C1 Single-Cell Whole Genome Sequencing workflow provides a comprehensive and reliable single-cell workflow to identify mutations in both regulatory and protein-coding regions of the genome and directly associate these variants to specific clonal populations.

Fluidigm’s new single-cell whole genome sequencing workflow is optimized to provide robust amplification of DNA templates from 96 single cells without compromising fidelity. By reducing reaction volumes, it saves >75% in sequencing preparation costs. The output yield from each single cell is sufficient to conduct multiple rounds of DNA sequencing. With the release of this workflow, Fluidigm now has a complete suite of sequencing protocols that enable researchers to easily pursue whole genome, whole exome, and targeted sequencing from an individual cell.

Cancer Webinar: Striving for a Cure, One Cell at a Time

Fluidigm recently hosted a cancer webinar that focused on Single-Cell DNA sequencing as a tool to discover predictive markers for cancer onset, metastasis, and therapy response. Featured in the webinar are some of the world’s most notable experts: Oxford University’s Paresh Vyas, whose field of expertise is predictive markers for AML and McGill University’s Jiannis Ragoussis, whose research focuses on constructing predictive biomarker panels for breast cancer treatment.

To view the recorded webinar and learn more, please go to: https://www.fluidigm.com/articles/striving-for-a-cancer-cure-one-cell-at-a-time.


The Fluidigm C1 system is based on the company’s innovative microfluidic technology that enables researchers to rapidly and reliably isolate, process, and profile individual cells for genomic analysis. The Single-Cell Whole Genome Sequencing workflow is the sixth application enabled on the C1 system, which also supports targeted gene expression, mRNA sequencing, miRNA expression profiling, targeted DNA sequencing, and whole exome sequencing.

Source: Business Wire